Coding

Part:BBa_K3515010:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-29)


Vitamin D Receptor Binding Protein with cysteine modification(s) to bind to a biosensor.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a Cysteine for immobilization (S224C) opposite to the active site region. Removed C34S, C120L, C169V, C201S, C235F, and C242T to ensure only one cysteine would bind to an immobilization linker arm. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (J03258.1) along with our own modifications.

References

Choi, M., Yamada, S. and Makishima, M., 2011. Dynamic and ligand-selective interactions of vitamin D receptor with retinoid X receptor and cofactors in living cells. Molecular pharmacology, 80(6), pp.1147-1155.